The Caenorhabditis elegans Transgenic Toolbox
نویسندگان
چکیده
منابع مشابه
Effects of α-Synuclein Overexpression in Transgenic Caenorhabditis elegans Strains
The neural protein α-synuclein aggregates both in vivo and in vitro to form insoluble fibrils that are involved in Parkinson's disease pathogenesis. We have generated α-synuclein/fluorescent-protein fusion constructs overexpressed in muscle cells of the nematode, Caenorhabdtis elegans. Green Fluorescent Protein (GFP) variants, Cerulean (C) or Venus (V), were fused to the C-terminus of human α-s...
متن کاملCreation of low-copy integrated transgenic lines in Caenorhabditis elegans.
In Caenorhabditis elegans, transgenic lines are typically created by injecting DNA into the hermaphrodite germline to form multicopy extrachromosomal DNA arrays. This technique is a reliable means of expressing transgenes in C. elegans, but its use has limitations. Because extrachromosomal arrays are semistable, only a fraction of the animals in a transgenic extrachromosomal array line are tran...
متن کاملA growing molecular toolbox for the functional analysis of microRNAs in Caenorhabditis elegans.
With the growing number of microRNAs (miRNAs) being identified each year, more innovative molecular tools are required to efficiently characterize these small RNAs in living animal systems. Caenorhabditis elegans is a powerful model to study how miRNAs regulate gene expression and control diverse biological processes during development and in the adult. Genetic strategies such as large-scale mi...
متن کاملCaenorhabditis Elegans
Transparent, free-living nematode worm. Unsegmented body plan with full set of differentiated tissues (neural, endoderm, ectoderm and muscle). Genome size approximately 97 Mb, as five autosomes and one X sex chromosome. Fully sequenced genome, which comprises approximately 20 000 predicted genes. Defined cell lineage. Has made major contribution to studies of development, cell-to-cell signallin...
متن کاملCaenorhabditis elegans
The complete structure of the anterior sensory nervous system of the small nematode C . elegans has been determined by reconstruction from serial section electronmicrographs. There are 58 neurons in the tip of the head. Fiftytwo of these are arranged in sensilla. These include six inner labial sensilla, six outer labial sensilla, four cephalic sensilla and two amphids. Each sensillum consists o...
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ژورنال
عنوان ژورنال: Genetics
سال: 2019
ISSN: 1943-2631
DOI: 10.1534/genetics.119.301506